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Carluke |
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Carluke
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If you found yerself in CARLUKE, Div and the dirt swans might take you in, feed you Stella and make you an honorary swan. For anti-Mullerian hormone (AMH) and [alpha]-smooth muscle actin (SMA) immunostaining, normal rabbit serum was used; for all other antibodies, normal swine serum was used (Scottish Antibody Production Unit, Carluke, Scotland, UK). For immunolocalization studies we used the following antisera: ER[beta] was immunolocalized with a polyclonal antibody raised in sheep (S40) to a peptide specific for the hinge (D) domain of human ER[beta] conjugated to keyhole limpet hemocyanin (Affinity, Exeter, UK) according to standard methods (Diagnostic Services Scotland, Carluke, UK) as previously described (9). |
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