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Kumasi |
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KumasiSecond-largest city in Ghana, capital of Ashanti region, situated 160 km/100 mi from the coast within the forest zones and 180 km/112 mi northwest of the capital and port of Accra; population (2000) 1,171,300. Kumasi is a major centre of Ghana's transport system, with an airport, as well as road and rail communications. It trades in cocoa, rubber, and cattle and its market is one of the largest in Africa. Food processing, brewing, logging, lumber, and tourism are the main industries. The Ghana National Culture Centre, which includes a zoo, an art gallery, and an open-air theatre, the palace of the Ashanti king, and the Ghanian University of Science and Technology are in Kumasi. HistoryFrom the late 17th century until 1901, when it was absorbed into the British Gold Coast Colony, Kumasi was capital of the Ashanti confederation.
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| Gels were stained with Coomassie brilliant blue R-250 or transferred to nitrocellulose membranes by using a Semiphor unit (Hoefer Scientific Instruments, San Francisco, CA, USA). After the image analysis, gels containing the additional load of proteins unlabeled proteins (gel 4; Figure 1) were stained with Colloidal Coomassie Brilliant Blue G (Acros Organics, Geel, Belgium) and matched to the fluorescent 2D-DIGE images. It is interesting to note that each of two renowned illustrations of Asante regalia taken by British troops contains a pectoral stripped of suspension materials; see "Ashantee golden ornaments and trophies," from the Illustrated London News, 1874; and "Trophies from Coomassie," The Graphic, April 11, 1874, reproduced in Fagg 1974:44-46, figs. |
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