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nucleotide |
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nucleotide
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c) A, C, G, and T refer to the four deoxynucleotides that DNA comprises. In the first step to assay for recombination, DNA (125,250, 500, and 1,000 ng) was suspended in a 50- [micro] l solution containing 200 [micro] M deoxynucleotides, 50 mM KCl, 10 mM Tris, pH 8. The BioArray(TM) CGH Labeling System is optimized for the preparation of Cy3 and Cy5 labeled DNA and provides all necessary reagents for random primed labeling, including primers, Klenow DNA polymerase, and Enzo's proprietary Cy3 and Cy5 labeled deoxynucleotides. |
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