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constant
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constant

In mathematics, a fixed quantity or one that does not change its value in relation to variables. For example, in the algebraic expression y2 = 5x − 3, the numbers 3 and 5 are constants. In physics, certain quantities are regarded as universal constants, such as the speed of light in a vacuum.



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In summary, this method is based on the experimental finding that the apparent Michaelis constant values depend on the concentration of the second substrate.
This paper presents an undergraduate laboratory exercise that allows for the experimental determination of the typical enzyme-kinetics parameters [the Michaelis constant (KM), the maximum rate of the enzyme-catalyzed reaction (Vmax), the turnover number (kcat), and the catalytic efficiency of the enzyme system at low substrate concentrations (kcat/KM)] using a standard benchtop multi-cell UV-vis spectrophotometer.
 
 
 
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