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acridine |
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acridine
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Diagnosis is
routinely made by conventional microscopy (Giemsa-stained thick blood
smear) or fluorescence microscopy after concentration of blood in
capillary tubes and staining with acridine orange (quantitative buffy
coat [QBC] analysis) (3). 08 N HCl for 30 sec and
then stained with 6 mg/L of purified acridine orange (AO; Molecular
Probes, Eugene, OR, USA) in a phosphate-citrate buffer, pH 6. Apoptotic cells in live, transparent zebrafish can be
visualized by acridine orange staining without complicated processing. |
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