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polymerase chain reaction |
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polymerase chain reaction
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| Annealing temperature was 55[degrees]C; cyclic scheme and further conditions were as reported previously (6). Amplifications were performed with AmpliTaq Gold PCR Master Mix (Applied Biosystems, Foster City, CA, USA) following the manufacturer's recommendations and a 50[degrees]C annealing temperature. Up to an annealing temperature of 375 K, relatively small changes in the microstructure of the specimens occur. |
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