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autoradiography
(redirected from autoradiograph)

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autoradiography

In biology, a technique for following the movement of molecules within an organism, especially a plant, by labelling with a radioactive isotope that can be traced on photographs. It is commonly used to study photosynthesis, where the pathway of radioactive carbon dioxide can be traced as it moves through the various chemical stages.



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Autoradiographs were developed from the hybridized filters with Kodak X-Omat AR x-ray film (Eastman Kodak Co.
In one method, the researchers bound the ATR to carbohydrate beads, added radiolabeled DNA, washed the beads three times to eliminate unbound DNA, and then visualized the ATR-bound DNA by autoradiograph.
We analyzed the autoradiographs containing the GLUT 1 and GLUT 4 blots by scanning densitometry (Gel Pro Analyzer 2.
 
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