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antiseptic |
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antisepticAny substance that kills or inhibits the growth of micro-organisms. The use of antiseptics was pioneered by Joseph Lister. He used carbolic acid (phenol), which is a weak antiseptic; antiseptics such as TCP are derived from this. Lister's work was extended by surgeons such as William Cheyne, professor of surgery at Kings College Hospital, London, and author of Antiseptic Surgery (1882). How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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Upon harvesting, 25 [micro]L of broth was added to 2 mL of brain-heart infusion broth, incubated for 4 h, and plated on cetrimide, Chapman, and bile-esculin-acid agar containing 10 mg/L of vancomycin (after an enrichment step of 18 h in broth containing 1 mg/L of vancomycin) to detect Pseudomonas aeruginosa, Staphylococcus aureus, and vancomycin-resistant enterococci, respectively. For feces, aliquots were plated on Chromagar, Cetrimide (Bio-Merieux), and Chapman agar for detection of yeasts, Pseudomonas aeruginosa, and S. The membranes were placed on Pseudomonas cetrimide fucidin cephalosporin (CFC) agar (Oxoid) and incubated at 30[degrees]C for 48 h. |
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