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chemiluminescence |
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chemiluminescenceThe emission of light from a substance as a result of a chemical reaction (rather than raising its temperature). See luminescence. How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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Scientists aren't sure why we glow, but they suspect it's because of chemoluminescence, or light-producing chemical reactions similar to those in fireflies. The antigen-antibody complexes were visualized using the chemoluminescence substrate SuperSignal West Pico (Pierce, Rockford, IL) as recommended by the manufacturer. 1%, and 0%) in 2x SSPE, the membranes were incubated for 2 minutes with the enhanced chemoluminescence (ECL) detection reagents (Amersham Life Science, s`Hertogenbosch, the Netherlands), and then were exposed to an ECL hyperfilm (Amersham Life Science) for 30 minutes and overnight to visualize the bound probe. |
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