Another method that combines immunoaffinity chromatography with specific antibodies for cleavage products has been successful in detecting BoNT B in some foods at lower detection limits than the mouse bioassay (10).

The insulin-producing cells also secreted C-peptide, a cleavage product generated in islet cells during the secretion of insulin.

1-transfected cell lysate was used as the negative control, e, Inhibition of WNV-Cp-induced apoptosis by a dominant negative (DN) caspase-9 plasmid (DN Casp9) was assayed with equal amount of cell lysates from co-transfection of pcWNV-Cp-DJY (Cp-DJY) or pcWNV-CpWT (CpWT); an expression level of pro-caspase-9 cleavage products (35-37 kDa) was compared to 3'-terminal deletion mutant, pcWNV-Cp[DELTA]3'(Cp[DELTA]3') and pcDNA3.