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electrophoresis |
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electrophoresisThe diffusion of charged particles through a fluid under the influence of an electric field. It can be used in the biological sciences to separate molecules of different sizes, which diffuse at different rates. In industry, electrophoresis is used in paint-dipping operations to ensure that paint reaches awkward corners. How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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| The Agilent 2100 Bioanalyzer system (Agilent Technologies, Waldbronn, Germany) was used to separate 1 [micro]L of PCR product electrophoretically. Equal amounts (20 lag of protein) of each sample were electrophoretically separated on a 10% SDS polyacrylamide gel and electro-transferred to a PVDF (polyvinylidene difluoride) membrane. We then electrophoretically transferred([double dagger])([double dagger]) the proteins at 300 mA for 60 minutes onto to polyvinylidene fluoride (PVDF) microporous membrane (Immobilon-P Transfer Membrane). |
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