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spectrum |
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spectrumIn physics, the pattern of frequencies or wavelengths obtained when electromagnetic radiations are separated into their constituent parts. Visible light is part of the electromagnetic spectrum and most sources emit waves over a range of wavelengths that can be broken up or ‘dispersed’; white light can be separated (for example, using a triangular prism) into red, orange, yellow, green, blue, indigo, and violet. The visible spectrum was first studied by English physicist Isaac Newton, who showed in 1666 how white light could be broken up into different colours. How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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5) is performed on the emission spectrum from a reference solution with known concentration of fluorophore and the emission spectrum from a suspension of microspheres with immobilized FITC. It is widely accepted that describing a measured x-ray peak by a Gaussian distribution is a sufficient approximation to derive accurate x-ray intensities from an x-ray emission spectrum measured with an EDS. It has been found that emission spectrum matching between a reference solution and an analyte and normalization by the corresponding extinction coefficient are required for quantifying fluorescence signals using flow cytometers. |
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