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| Semithin (1 to 2 pm) sections were cut with glass knives on an ultramicrotome and stained with toluidine blue and basic fuchsin for light microscopic examination with bright-field and differential interference contrast optics. Fuchsin fluorescence and autofluorescence in Cryptosporidium, Isospora and Cyclospora oocysts. They were stained with Kinyoun's carbol fuchsin modified acid-fast stain and examined by oil immersion microscopy. |
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