sequencing - Hutchinson encyclopedia article about sequencing Printer Friendly
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sequencing

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sequencing

In biochemistry, determining the sequence of chemical subunits within a large molecule. Techniques for sequencing amino acids in proteins were established in the 1950s, insulin being the first for which the sequence was completed. The Human Genome Project was set up to determine the sequence of the 3 billion base pairs within human DNA. In April 2003, scientists announced the completion of the mapping of the whole human genome.

Scientists are now able to routinely determine the genome sequence of organisms ranging from bacteria to plants and mammals.



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Other topics include sequence data analysis, real-time DNA sequencing, a single DNA molecule barcoding method with applications in DNA mapping and molecular halpotyping, optical sequencing, the multiplex sequencing of paired end ditags for transcriptome and genome analysis, microRNA discovery and expression profiling, and ethical issues about personal genome information.
We report results of further characterization of this virus, including growth kinetics in cell culture and full-length genome sequencing and genetic characterization, which show it to be distinct from DUGV.
The most widely used technology for DNA sequencing is a capillary electrophoresis-based (CE) system employing the Sanger method.
 
 
 
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